Heteroplasmy was detected in the formosan macaque, domestic pig and domestic cats. modern method of animal species identification. USA.gov. Recent estimates of the number of species range from 10 to 100 million (Wilson 1998). Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. which is in the field of taxonomy and the identification of species. Cytochrome b gene for species identification of the conservation animals Hsieh, H. and Chiang, H. and Tsai, L. and Lai, S. and Huang, N. and Linacre, A.M.T. Background: Species identification is an important process to identify the origin of meat, adulteration and for cooked and processed meat. The cytochrome b gene was successfully amplified in all Molossid and Vespertilionid species except for Eptesicus, for which we designed a new reverse primer. Traditionally the cytochrome b (cyt b) gene was used for species identification, but in 2003 the cytochrome c oxidase subunit 1 (CO1) gene was introduced … All sequences were submitted to the GenBank including new species which were not observed in the databases. Material and Methods Eight polymorphic partial cytochrome b sequences Species Identification and PCR‐RFLP Analysis of Cytochrome b Gene in Cod Fish (Order Gadiformes) Products Tetsuya Akasaki Authors Akasaki, Tomonaga, and Sato are with Central Customs Laboratory, Ministry of Finance, 6‐3‐5 Kashiwanoha, Kashiwashi, … Cytochrome b is a component of respiratory chain complex III [2, 3] . 2009 Mar;3(2):67-73. doi: 10.1016/j.fsigen.2008.10.005. As an alternative to direct DNA sequencing of PCR products, random PCR-RFLP is an efficient technique to discriminate between species. September 2007] MEAT SPECIES IDENTIFICATION BY MULTIPLEX PCR 881 1 (5'-CTC AGA TTC ACT CGA CGA GGG TAG TA-3') were used. The cytochrome b gene of mitochondrial DNA (mtDNA) has been used in species identification , , , , and in taxonomic and phylogenetic studies . Forensic Science International, 122 (1). The high copy number of mtDNA compared to nuclear DNA makes this locus ideal for analysis from highly degraded DNA. Nakaki S, Hino D, Miyoshi M, Nakayama H, Moriyoshi H, Morikawa T, Itohara K (2007) Study of animal species (human, dog and cat) identification using a multiplex single-base primer extension reaction in the cytochrome b gene. Kumar VP, Shukla M, Rajpoot A, Thakur M, Nigam P, Kumar D, Mehta AK, Goyal SP. Because adulteration of the drug with substances from other animals such as horses, cattle, and pigs has been found, we examined PCR methods based on the sequence of the cytochrome b gene for source species identification. Electrophoresis. In this paper, we studied the section Nigri based on a 402-bp segment of the mt cytochrome b gene. Epub 2016 May 6. Cytochrome b/b6 is an integral membrane protein of approximately 400 amino acid residues that probably has 8 transmembrane segments. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. modern method of animal species identification. 3. Zhu K, Suttner B, Pickering A, Konstantinidis KT, Brown J. The sequences derived were used to identify the … Interspecies-specific DNA sequence polymorphisms in the mitochondrial cytochrome b gene … Length of CytB gene is 1140 bp and has some Sequences of suspect rhinoceros horns were compared with the sequences registered in GenBank. Cattle primer was used for the amplification of buffalo cytochrome b gene. The control species used included domestic cats, domestic dogs, domestic sheeps, domestic cattles, domestic pigs and humans. Abstract. https://doi.org/10.1016/S0379-0738(01)00403-0. It encodes the β‐chain of flavocytochrome b 245 (also called as gp91 phox or NOX2). Species identification of rhinoceros horns using the cytochrome b gene Hsing-Mei Hsieha, Li-Hung Huangb, Li-Chin Tsaia, Yi-Chen Kuob, Hsien-Huei Menga, Adrian Linacrec, James Chun-I Leea,* aDepartment of Forensic Science, Central Police University, 56 Shu-Jen Road, Kwei-San, Taoyuan 33334, Taiwan, ROC bForensic Science Laboratory, Central Police University, 56 Shu … Number of species range from 10 to 100 million ( Wilson 1998 ) unambiguous identification of fish species all. Obtained from domestic camel, buffalo and sheep used, as the value of evidence... A reference library after analysis of the NADPH oxidase complex in phagocytes, such as granulocytes monocytes... Software is designed to automatically identify the closest sequences from a reference library after analysis of all known …. I, Bocianowski J. Int J Mol Sci conservation animals restriction enzymes are HaeIII and HinfI, which 4-base! 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